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sRANK Ligand (soluble Receptor Activator of NFkB Ligand)

 Product Description:

RANKL and RANK are members of the TNF superfamily of ligands and receptors that play an important role in the regulation of specific immunity and bone turnover. RANK (receptor) was originally identified as a dendritic cell-membrane protein, which, by interacting with RANKL, augments the ability of dendritic cells. These dendritic cells then stimulate naïve T-cell proliferation in a mixed lymphocyte reaction, promote the survival of RANK + T-cells, and regulate T-cell-dependent immune response. RANKL, which is expressed in a variety of cells, including osteoblasts, fibroblasts, activated T-cells and bone marrow stromal cells, is also capable of interacting with a decoy receptor called OPG. Binding of soluble OPG to sRANKL inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage. Human RANKL is reactive on murine cells.

Technical Specifications:

  • Species: Human
  • Expression: E. coli Cell Expressed
  • Activity: Typically 10-25 ng/ml ED50
  • Purity: ≥98%
  • Endotoxin: <1.0 EU/μg
  • Molecular Mass: 20.0 kDa
  • Formulation: 5 mM Sodium Phosphate, pH 7.6 + 75 mM NaCl
  • Country of Origin: USA

Purity Confirmation:

This was determined by SDS-PAGE gel and HPLC analysis.

Activity Assay:

Determined by its ability to induce NF-κB in RAW264.7 cells in the absence of any cross-linking. The expected ED50 for this effect is 10.0-25.0 ng/ml.

AA Sequence:

MEKAMVDGSW LDLAKRSKLE AQPFAHLTIN
ATDIPSGSHK VSLSSWYHDR GWAKISNMTF
SNGKLIVNQD GFYYLYANIC FRHHETSGDL
ATEYLQLMVY VTKTSIKIPS SHTLMKGGST
KYWSGNSEFH FYSINVGGFF KLRSGEEISI
EVSNPSLLDP DQDATYFGAF KVRDID

Reconstitution Buffer:

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex.

Storage:

For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% HSA) and store in working aliquots at -20°C to -80°C.

Cat #: Size Price
HZ-7227 10 µg $201
HZ-7233 50 µg $643
HZ-7230 100 µg $1,071
HZ-7231 500 µg $3,214
HZ-7232 1000 µg $5,356

XKine™ proteins are expressed in several expression systems

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