Products : Recombinant Human SHH (Sonic Hedgehog)

Products > Product Cytokines > SHH

Recombinant Human SHH (Sonic Hedgehog)

 Product Description:

• Endotoxin-free
• Animal-derived product free
• Lyophilized and Carrier Free (CF)

Xeno-free Recombinant Human SHH (Sonic Hedgehog)^HuXp protein is the orthologue of drosophila hedgehog (Hh) and plays a critical role in vertebrate limb development. SHH gene encodes for a 45 kDa protein that undergoes autoproteolysis activated by its own C-terminal domain. Furthermore, the C-terminal domain also carries out cholesterol transferase activity. This auto-cleavage results in the formation of a 174 amino acid N-terminal product with covalently linked cholesterol moiety and a palmitoyl modification. The presence of the cholesterol and palmitoyl moieties is required for the activity of the mature SHH protein. These modifications are found in SHH derived from human cells, but are absent in bacterially expressed SHH. The N-terminal (active) form of SHH shares 98% aa homology to mouse, rat, canine, porcine and chicken SHH. It has been associated with tissue regeneration following injury and development of certain cancers in adults.

Technical Specifications:

• Species: Human
• Expression: HEK293 Cell Expressed
• Activity: Typically <350 ng/mL EC50
• Purity: >90%
• Endotoxin: <0.5 EU/μg
• Molecular Mass: 20-24 kDa
• Formulation: 10mM Tris pH 7.4 + 100mM NaCl + 1% CHAPS

Purity Confirmation:

The protein was resolved by SDS-polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Activity Assay:

The activity was determined by ability to induce alkaline phosphatase production in C3H10T1/2 mouse embryonic fibroblast cells.

Reconstitution Buffer:

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile water containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

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