Products : Recombinant Human PDGFbb

Products > Product Cytokines > PDGFbb

Recombinant Human PDGFbb (Platelet-Derived Growth Factor bb)

Product Description:

• Endotoxin-free
• Animal-derived product free
• Lyophilized and Carrier Free (CF)
• High Stability

Xeno-free Recombinant Human PDGFbb^HuXp is a growth factor that promotes blood vessel formation, mitogenesis, chemotaxis, etc. The PDGF family members (four homodimers and one heterodimer) are secreted, disulfide-linked dimeric glycoproteins that regulate their cellular functions through interaction with PDGFR ( and ) receptors. PDGFbb is synthesized, stored, and released by alpha granules of platelets. It is also known as PDGF-2, becaplermin, or GDGF. Dysregulation in PDGF signaling has been shown to be associated with tumorigenesis and progression of cancer. Recombinant PDGFbb is used in treatment of chronic ulcers and to speed healing in surgical procedures.

Technical Specifications:

• Species: Human
• Expression: HEK293 Cell Expressed
• Activity: Typically ≤ 3 ng/mL EC50
• Purity: >95%
• Endotoxin: <1 EU/μg
• Molecular Mass: 29 to 32 kDa, homodimer, glycosylated
• Formulation: 50mM Acetate pH 3.5 + 500mM NaCl

Purity Confirmation:

The protein was resolved by SDS-polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Activity Assay:

The activity was determined by the dose- dependent stimulation of the proliferation of 3T3 cells.

Reconstitution Buffer:

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 10 mM HOAc containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Synonyms: Platelet-Derived Growth Factor-BB, Glioma-derived growth factor (GDGF), Osteosarcoma-derived Growth Factor (ODGF) Platelet-Derived Growth FactorAA, Platelet-Derived Growth Factor AA,