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PDGFbb (Platelet-Derived Growth Factor bb)

Product Description:

PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs, PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types, including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet α-granules, and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is a high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. Recombinant Human PDGF-BB is a 24.3 kDa disulfide-linked homodimer of two β chains (218 total amino acids).

Technical Specifications:

  • Species: Human
  • Expression: E. coli Cell Expressed
  • Activity: Typically 1.0-3.0 ng/mL ED50
  • Purity: ≥98%
  • Endotoxin: <1.0 EU/μg
  • Molecular Mass: 24.3 kDa
  • Formulation: 10mM Acetic Acid
  • Country of Origin: USA

Purity Confirmation:

This was determined by SDS-PAGE gel and HPLC analysis.

Activity Assay:

Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells

AA Sequence:

SLGSLTIAEP AMIAECKTRT EVFEISRRLI
DRTNANFLVW PPCVEVQRCS GCCNNRNVQC
RPTQVQLRPV QVRKIEIVRK KPIFKKATVT
LEDHLACKCE TVAAARPVT

Reconstitution Buffer:

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex.

Storage:

For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% HSA) and store in working aliquots at -20°C to -80°C.

Cat #: Size Price
HZ-7091 2 µg $82
HZ-7092 10 µg $201
HZ-7093 100 µg $803
HZ-7094 250 µg $1,406
HZ-7095 500 µg $2,410
HZ-7096 1000 µg $4,017

XKine™ proteins are expressed in several expression systems

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