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Heregulin beta 1

Product Description:

Neuregulin/Heregulin is a family of structurally related polypeptide growth factors derived from alternatively spliced genes (NRG1, NRG2, NRG3 and NRG4). To date, there are over 14 soluble and transmembrane proteins derived from the NRG1 gene. Proteolytic processing of the extracellular domain of the transmembrane NRG1 isoforms releases soluble growth factors. HRG1-β1 contains an Ig domain and an EGF-like domain; the latter is necessary for direct binding to receptor tyrosine kinases erb3 and erb4. This binding induces erb3 and erb4 heterodimerization with erb2, stimulating intrinsic kinase activity that leads to tyrosine phosphorylation. Although HRG1-β1’s biological effects are still unclear, it has been found to promote motility and invasiveness of breast cancer cells, which may also involve up-regulation of expression and function of the autocrine motility-promoting factor (AMF). Recombinant Human Heregulinβ-1 (HRG1-B1) is a 7.5 kDa polypeptide consisting of only the EGF domain of heregulinβ-1 (65 amino acid residues).

Technical Specifications:

  • Species: Human
  • Expression: E. coli Cell Expressed
  • Activity: Typically ≤0.5 ng/mL ED50
  • Purity: >98%
  • Endotoxin: <1.0 EU/μg
  • Molecular Mass: 7.5 kDa
  • Country of Origin: USA

Purity Confirmation:

This was determined by SDS-PAGE gel and HPLC analysis.

Activity Assay:

Determined by the dose-dependent stimulation of the proliferation of human MCF-7 cells.

AA Sequence:

SHLVKCAEKE KTFCVNGGEC FMVKDLSNPS
RYLCKCPNEF TGDRCQNYVM ASFYKHLGIE
FMEAE

Reconstitution Buffer:

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex.

Storage:

For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% HSA) and store in working aliquots at -20°C to -80°C.

Cat #: Size Price
HZ-7034 10 µg $82
HZ-7035 50 µg $201
HZ-7036 100 µg $309
HZ-7037 250 µg $541
HZ-7038 500 µg $927
HZ-7039 1000 µg $1,545

XKine™ proteins are expressed in several expression systems

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