TNF alpha (Tumor necrosis factor alpha)
- Animal-derived product free
- Available in Bulk
- Expressed as Native Trimer Form
- Lyophilized and Carrier Free (CF)
Xeno-free TNF alpha is expressed in human 293 cells and has been shown by gel filtration to be predominantly a non-covalently linked homotrimer, with a molecular mass of 51 kDa. The E. coli form is not a trimer. The cytokine is produced in a human cell express ion system with serum free, chemically defined media.
- Species: Human
- Expression: HEK293 Cell Expressed
- Activity: Typically ≤1 ng/ml EC50
- Purity: >95%
- Endotoxin: <I EU/µg
- Molecular Mass: 17 kDa, non-disulfide bonded homotrimer, glycosylated
- Formulation 1x PBS
The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.
The activity was determined by the dose- dependent cytotoxity of the TNF alpha sensitive cell line L-929 in the presence of Actinomycin D.
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile
1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).
Synonyms: Tumor Necrosis Factor, TNFSF2, Cachectin, Differentiation-inducing factor (DIF), Necrosin, Cytotoxin