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Products > Product Cytokines > TNF alpha

TNF alpha  (Tumor necrosis factor alpha)

Product Description:

  • Endotoxin-free
  • Animal-derived product free
  • Available in Bulk
  • Expressed as Native Trimer Form
  • Lyophilized and Carrier Free (CF)

Xeno-free TNF alphaHuXp is expressed in human 293 cells and has been shown by gel filtration to be predominantly a non-covalently linked homotrimer, with a molecular mass of 51 kDa. The E. coli form is not a trimer. The cytokine is produced in a human cell express ion system with serum free, chemically defined media.

Technical Specifications:

  • Species: Human
  • Expression: HEK293 Cell Expressed
  • Activity: Typically ≤1 ng/mL EC50
  • Purity: >95%
  • Endotoxin: <I EU/µg
  • Molecular Mass: 17 kDa, non-disulfide bonded homotrimer, glycosylated
  • Formulation 1x PBS

Purity Confirmation:

The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Activity Assay:

The activity was determined by the dose- dependent cytotoxity of the TNF alpha sensitive cell line L-929 in the presence of Actinomycin D.

Reconstitution Buffer:

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile
1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Synonyms: Tumor Necrosis Factor, TNFSF2, Cachectin, Differentiation-inducing factor (DIF), Necrosin, Cytotoxin

Cat #: Size Price
HZ-1014 10 µg $106
HZ-1201 100 µg $690
HZ-1202 1000 µg $4,270

Human Cell Expressed

Animal Component-Free, Xeno-Free Production