Products : Recombinant Human VEGF165
Recombinant Human VEGF165 (Vascular Endothelial Growth Factor 165)
- Animal-derived product free
- Lyophilized and Carrier Free (CF)
- Authentic Glycosylated Homodimer
Xeno-free Recombinant Human VEGF 165is expressed in human 293 cells as a homodimeric glycoprotein with an apparent molecular mass of 45 kDa. The E. coli expressed protein is a monomer-dimer mixture with a molecular mass 18 to 34 kDa. The cytokine is produced in a human cell expression system with serum free, chemically defined media. VEGF, also known as vascular permeability factor (VPF) or vasculotropin, is a homodimeric 34-45 kDa, heparin-binding glycoprotein with potent angiogenic, mitogenic, and vascular permeability enhancing activities specific for endothelial cells.
- Species: Human
- Expression: HEK293 Cell Expressed
- Activity: Typically ≤15 ng/mL EC50
- Purity: >95%
- Endotoxin: <1 EU/µg
- Molecular Mass: 45 kDa, homodimer, glycosylated
- Formulation: lx PBS
The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.
The activity was determined by the dose-dependent stimulation of the proliferation of HUVEC cells (Human Umbilical Vein Endothelial Cells).
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant serum albumin (HSA).
Synonyms: Vascular Endothelial Growth Factor, VPF, Folliculostellate cell-derived growth factor, Glioma-derived endothelial cell mitogen Folliculostellate cell derived growth factor, Glioma derived endothelial cell mitogen