Products : Recombinant Human VEGF165

Products > Product Cytokines > VEGF165

Recombinant Human VEGF165 (Vascular Endothelial Growth Factor 165)

Product Description:

• Endotoxin-free
• Animal-derived product free
• Lyophilized and Carrier Free (CF)
• Authentic Glycosylated Homodimer

Xeno-free Recombinant Human VEGF 165is expressed in human 293 cells as a homodimeric glycoprotein with an apparent molecular mass of 45 kDa. The E. coli expressed protein is a monomer-dimer mixture with a molecular mass 18 to 34 kDa. The cytokine is produced in a human cell expression system with serum free, chemically defined media. VEGF, also known as vascular permeability factor (VPF) or vasculotropin, is a homodimeric 34-45 kDa, heparin-binding glycoprotein with potent angiogenic, mitogenic, and vascular permeability enhancing activities specific for endothelial cells.

Technical Specifications:

• Species: Human
• Expression: HEK293 Cell Expressed
• Activity: Typically ≤15 ng/mL EC50
• Purity: >95%
• Endotoxin: <1 EU/µg
• Molecular Mass: 45 kDa, homodimer, glycosylated
• Formulation: lx PBS

Purity Confirmation:

The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Activity Assay:

The activity was determined by the dose-dependent stimulation of the proliferation of HUVEC cells (Human Umbilical Vein Endothelial Cells).

Reconstitution Buffer:

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant serum albumin (HSA).

Synonyms: Vascular Endothelial Growth Factor, VPF, Folliculostellate cell-derived growth factor, Glioma-derived endothelial cell mitogen Folliculostellate cell derived growth factor, Glioma derived endothelial cell mitogen