Products : Recombinant Human VEGF121

Products > Product Cytokines > VEGF121

Recombinant Human VEGF121 (  Vascular Endothelial Growth Factor 121 )

Product Description:

• Endotoxin-free
• Animal-derived product free
• Lyophilized and Carrier Free (CF)
• Glycosylated Dimer – Trimer

Xeno-free Recombinant Human VEGF121 is expressed in human 293 cells as a glycosylated cytokine with an apparent molecular mass of 37 kDa as dimer and 50 kDa as trimer. Production in human 293 cells offers authentic glycosylation, contributing to stability in cell growth media. The cytokine is produced in a human cell expression system with serum free, chemically defined media. VEGF121 is a potent growth and angioenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis amd vascular permeability.

Technical Specifications:

• Species: Human
• Expression: HEK293 Cell Expressed
• Activity: Typically ≤15 ng/mL EC50
• Purity: >95%
• Endotoxin: <1 EU/µg
• Molecular Mass: 37 kDa, homodimer; 50 kDa, homotrimer, glycosylated
• Formulation: 1x PBS

Purity Confirmation:

The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Activity Assay:

The activity was determined by the dose- dependent stimulation of the proliferation of HUVEC cells (Human Umbilical Vein Endothelial Cells).

Reconstitution Buffer:

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Synonyms: Vascular Endothelial Growth Factor121, VPF Vascular Endothelial Growth Factor 121