Products : Recombinant Human IL-6

Products > Product Cytokines > IL-6

Recombinant Human IL-6  (Interleukin 6)

Product Description:

• Endotoxin-free
• Animal-derived product free
• Lyophilized and Carrier Free (CF)
• Authentic Glycosylation

Xeno-free Recombinant Human IL-6^HuXp is produced in human 293 cells as a monomeric glycoprotein with an apparent molecular mass of 21 to 29 kDa. This broad molecular mass is attributable to glycosylation, which is absent when this cytokine is expressed in E. coli. Glycosylation contributes to stability in cell growth media and other applications. IL-6 is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells, and keratinocytes, IL-6 has diverse biological functions.

Technical Specifications:

• Species: Human
• Expression: HEK293 Cell Expressed
• Activity: Typically ≤1 ng/mL EC50
• Purity: >95%
• Endotoxin: <1 EU/µg
• Molecular Mass: 21 and 29 kDa, monomer, glycosylated
• Formulation: 1x PBS

Purity Confirmation:

The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Activity Assay:

The activity was determined by the dose- dependent stimulation of the proliferation of 7TD1 cells (mouse hybridoma cell line).

Reconstitution Buffer:

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Synonyms: 26 kDa protein, IFN-&beta;2, B cell differentiation factor (BCDF), BSF-2, HPGF, HSF, MGI-2 IFN&beta;2, IFN &beta;2, BSF2, BSF 2, MGI2, MGI 2