Products : Recombinant Human IL-10

Products > Product Cytokines > IL-10

Recombinant Human IL-10 (Interleukin 10)

Product Description:

• Endotoxin-free
• Animal-derived product free
• Lyophilized and Carrier Free (CF)
• High Activity
• Authentic Glycosylation

Xeno-free Recombinant Human IL-10^HuXp is expressed in human 293 cells as a non-covalent homodimeric glycoprotein with an apparent molecular mass of 17 kDa. This cytokine is produced in a serum-free, chemically defined media. IL-10 is produced by a variety of mammalian cell types which includes T cells, B cells, macrophages, and keratinocytes monocytes. It is an anti-inflammatory cytokine with multiple, pleiotropic effects in inflammation and immunoregulation. Glycosylation contributes to stability in cell growth media and other applications. The cytokine is greater than 95% pure.

Technical Specifications:

• Species: Human
• Expression: HEK293 Cell Expressed
• Activity: Typically ≤1.5ng/mL EC50
• Purity: >95%
• Endotoxin: <1 EU/μg
• Molecular Mass: 17 kDa, non-disulfide bonded homodimer, glycosylated
• Formulation: 10mM Tris-HCl pH 7.4 + 150mM NaCl

Purity Confirmation:

The protein was resolved by SDS-polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Activity Assay:

The activity was determined by the dose-dependent stimulation of the proliferation of MC/9 cells (mouse mast cell line) in the presence of 200 pg/mL IL-4.

Reconstitution Buffer:

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Synonyms: B-TCGF, CSIF, TGIF IL 10, IL10, B TCGF, BTCGF