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Activin B

Product Description:

Activin B is a TGF-β family member that exhibits a wide range of biological activities, including regulation of embryogenesis, osteogenesis, hematopoiesis, reproductive physiology and hormone secretion from the hypothalamic, pituitary and gonadal glands. Activin B, like certain other members of the TGF-β family, signals through the ActRII receptor (Activin Receptor type II). Activins are homodimers or heterodimers of different β subunits. They are produced as precursor proteins with an amino terminal propeptide that is cleaved to release the C-terminal bioactive ligand. Human Activin B is a 25.6 kDa disulfide-linked homodimer consisting of two βB chains, each containing 115 amino acid residues.

Technical Specifications:

• Species: Human
• Expression: Hi-5 Insect Cell Expressed
• Activity: Typically ≤ 2.0 ng/ml ED50
• Purity: ≥95%
• Endotoxin: <1.0 EU/μg
• Molecular Mass: 25.6 kDa
• Formulation: 10 mM Sodium Citrate, pH 3.0 + 5% Trehalose
• Country of Origin: USA

Purity Confirmation:

This was determined by SDS-PAGE gel and HPLC analysis.

Activity Assay:

Determined by its ability to inhibit the proliferation of mouse MPC-11 cells.

AA Sequence:

Reconstitution Buffer:

Centrifuge the vial prior to opening. Reconstitute in 10mM Sodium Citrate, pH 3.0 to a concentration of 0.5 mg/ml. Do not vortex.

Storage:

For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% HSA) and store in working aliquots at -20°C to -80°C.