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Recombinant Human Myostatin-Propeptide

Product Description:

Mature myostatin is obtained by proteolytic processing of a biologically-inactive precursor protein, which contains an N-terminal propeptide of 243 amino acid residues. Myostatin-Propeptide exhibits high binding affinity for myostatin, and has been shown to be a potent inhibitor of myostatin. Over-expression of myostatin-propeptide in mice resulted in large increases (up to 200%) in skeletal muscle mass, similar to those observed in myostatin knockout mice. Recombinant Human Myostatin-Propeptide is a 27.8 kDa protein consisting of 244 amino acid residues.

Technical Specifications:

• Species: Human
• Expression: E. Coli Cell Expressed
• Activity: Typically 0.01-0.04 μg/mL ED50
• Purity: ≥98%
• Endotoxin: <1.0 EU/μg
• Molecular Mass: 27.8 kDa
• Formulation: 20mm HCl
• Country of Origin: USA

Purity Confirmation:

This was determined by SDS-PAGE gel and HPLC analysis.

Activity Assay:

Determined by its ability to neutralize the Myostatin inhibitory effect of murine MPC-11 cells. The expected ED50 is 0.01–0.04 μg/ml in the presence of 50 ng/ml Myostatin.

AA Sequence:

NGHDLAVTFPGPGEDGLNPFLEVKVTDTPK

Reconstitution Buffer:

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex.

Storage:

For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% HSA) and store in working aliquots at -20°C to -80°C.