Products : Recombinant Human IL-4

Products > Product Cytokines > IL-4

Recombinant Human IL-4  (Interleukin 4)

Product Description:

• Endotoxin-free
• Animal-derived product free
• Available in Bulk
• Higher Stability in Media
• Authentic Glycosylation
• Lyophilized and Carrier Free (CF)

Xeno-free Recombinant Human IL-4^HuXp also known as B cell-stimulatory factor-1. IL-4 is a monomeric, approximately 13 kDa and 19 kDa Th2 cytokine which shows pleiotropic effects during immune responses. Our IL-4 is produced in a human cell expression system with serum-free and chemically defined media. It has demonstrated greater stability under cell culture conditions making it ideal for efficient generation of Human Dendritic Cells. (See the time saving G4 protocol available at www.HumanZyme.com).

Technical Specifications:

• Species: Human
• Expression: HEK293 Cell Expressed
• Activity: Typically ≤0.6 ng/mL EC50
• Purity: >95%
• Endotoxin: <1 EU/µg
• Molecular Mass: 14 and 19 kDa, monomer, glycosylated
• Formulation: 1x PBS

Purity Confirmation:

The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Activity Assay:

The activity was determined by the dose- dependent simulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).

Reconstitution Buffer:

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Synonyms: BCGF, BCDF, B cell stimulating factor (BSF-1) BSF1, BSF 1