Products : Recombinant Human IL-2 beta

Products > Product Cytokines > IL-1 Beta

Recombinant Human IL-1 beta  (Interleukin 1 beta)

Product Description:

• Endotoxin-free
• Animal-derived product free
• Available in Bulk
• Authentic Glycosylation
• Lyophilized and Carrier Free (CF)

Xeno-free Recombinant Human IL-1 beta^HuXp expressed in human 293 cells has a very high activity with a typical EC50 of ≤0.1 ng/mL using a bioassay to measure stimulation of the proliferation of mouse D10S cells. In comparison with the E. coli expressed protein, IL-1 beta is 50% more potent promoting human CD4+ T cell differentiation into Th17 cells. This cytokine is important for inflammatory responses. It is also involved in a variety of cellular activities which includes cell differentiation, proliferation, and apoptosis. It is produced in a serum-free, chemically defined media.

Technical Specifications:

• Species: Human
• Expression: HEK293 Cell Expressed
• Activity: Typically ≤0.1 ng/mL EC50
• Purity: >95%
• Endotoxin: <1 EU/µg
• Molecular Mass: 18 and 25 kDa, monomer, glycosylated
• Formulation: 1x PBS

Purity Confirmation:

The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Activity Assay:

The activity was determined by the dose- dependent stimulation of the proliferation of mouse D10S cells.

Reconstitution Buffer:

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Synonyms: Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF) Lymphocyte activating factor (LAF)