Thermostable FGF Basic Supports a 2-Day Feeding Schedule As Shown by Biochemical and Cell Culture Analysis
FGF basic is a required component of stem cell culture media for maintaining cells in an undifferentiated state. Because FGF basic is unstable, daily media changes are needed. HumanZyme has developed a thermostable FGF basic (FGFbasic-TS) that supports a 2- day media change schedule, so no media changes are required over a weekend. This thermostable FGF basic was more stable than FGF basic in biochemical studies, and maintained cell growth, pluripotency and differentiation potential with a 2-day feeding schedule, as evaluated with pluripotency markers, karyotyping and a reporter gene assay.