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HumanKine® Recombinant Human G-CSF Is More Effective for Cell Growth Stimulation

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Cytokines are a group of proteins and polypeptides that organisms use as signaling molecules. Most cytokines are glycoproteins less than 30 kDa in size and bind to specific, high-affinity cell surface receptors. Due to their central role in the immune system, cytokines are involved in a variety of immunological, inflammatory and infectious diseases and widely used in research, diagnostics and therapeutics. Cytokines generally alter the gene expression pattern of the target cell which leads to changes in the rate of cell proliferation and/or in the state of cell differentiation. Currently, these proteins are predominantly produced in non-human cells (e.g. E. coli) and therefore lack authenticity due to the absence of physiologically relevant glycosylation. In addition, a number of important cytokines are not commercially available due to inadequate proteolytic processing, protein folding or other post-translational modifications that do not occur in the non-human cell expression systems.

HumanZyme has developed an efficient humancell based technology, HumaXpress®, for costeffective and scalable production of human cytokines. We have an expanding range of tag-free cytokines, including difficult-to-express members of the TGFβ superfamily. As demonstrated below, HumanZyme’s authentic cytokines can be used as highly preferred reagents for stem cell, cancer, inflammation research, and antibody development.

HumanKine G-CSF

Granulocyte colony-stimulating factor (G-CSF), is a hematopoietic growth factor that stimulates the development of committeed progenitor cells to neutrophils and enhances the functional activities of the mature end-cell. Currently, commercially available G-CSF proteins are produced in an E coli expression system and typically have a molecular mass of 19kDa. HumanZyme Inc. scientists have produced HumanKine G-CSF expressed in engineered human 293 cells. The protein is a glycosylated form with a molecular mass range of 22-25 kDa. HumanKine G-CSF can be cost-effectively produced at large scale.

The bioactivity of HumanKine G-CSF was determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells. G-CSF expressed in E. coli was compared to the human cell expressed version HumanKine G-CSF. The results indicate that HumanKine G-CSF is 4-5 fold more active than the E coli expressed cytokine and is more effective for stimulating cell growth.

(View more information for product number HZ-1207, HZ-1172, and HZ-1081.)


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