Products : Recombinant Human TGF beta 3

Products > Product Cytokines > TGF beta 3

Recombinant Human TGF beta 3  (Transforming Growth Factor beta 3)

Product Description:

• Endotoxin-free
• Animal-derived product free
• Available in Bulk
• Lyophilized and Carrier Free (CF)
• High Activity

Xeno-free Recombinant Human TGF beta 3^HuXp is expressed from human 293 cells as a mature disulfide-linked homodimer with apparent molecular mass of 25 kDa. This cytokine is produced in a human cell expression system with serum-free, chemically defined media. The cytokine is greater than 95% pure.

Technical Specifications:

• Species: Human
• Expression: HEK293 Cell Expressed
• Activity: Typically ≤0.5 ng/mL EC50
• Purity: >95%
• Endotoxin: <1 EU/µg
• Molecular Mass: 25 kDa, homodimer, non-glycosylated
• Formulation: 50 mM NaOAc pH 3.7

Purity Confirmation:

The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Activity Assay:

The activity was determined by the dose- dependent inhibition of IL-4 induced proliferation of mouse HT-2 cells (BALB/c spleen activated by sheep erythrocytes in the presence of IL-2).

Reconstitution Buffer:

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 4 mM HCl containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Synonyms: Transforming Growth Factor-&beta;2, Glioblastoma-derived T cell suppressor factor, BSC-1, Cetermin, Polyergin TGFbeta3, Transforming Growth Factor&beta;2, Transforming Growth Factor &beta;2, BSC1, BSC 1