Products : Recombinant Human OSM

Products > Product Cytokines > OSM

Recombinant Human OSM (Oncostatin M)

Product Description:

• Endotoxin-free
• Animal-derived product free
• Lyophilized and Carrier Free (CF)
• High Stability
• Native Quaternary Structure

Xeno-free Recombinant Human Oncostatin M^HuXp is expressed in human 293 cells as a monomeric glycoprotein with an apparent molecular mass of 30 kDa. This higher molecular mass is due to glycosylation and is not seen in the E. coli expressed form of this cytokine (22kDa). Glycosylation contributes to stability in cell growth media and other applications. This product is produced in a human cell expression system with serum-free, chemically defined media.

Technical Specifications:

• Species: Human
• Expression: HEK293 Cell Expressed
• Activity: Typically ≤10 ng/mL EC50
• Purity: >95%
• Endotoxin: <1 EU/μg
• Molecular Mass: 30 kDa, monomer, glycosylated
• Formulation: 1x PBS

Purity Confirmation:

The protein was resolved by SDS-polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Activity Assay:

The activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).

Reconstitution Buffer:

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Synonyms: Oncostatin M OncostatinM, Oncostatin-M