Products : Recombinant Human M-CSF

Products > Product Cytokines > M-CSF

Recombinant Human M-CSF (Macrophage Colony-Stimulating Factor)

Product Description:

• Endotoxin-free
• Animal-derived product free
• Available in Bulk
• Lyophilized and Carrier Free (CF)
• Authentic Glycosylated Homodimer

Xeno-free Recombinant Human M-CSF^HuXp is expressed in human 293 cells and has an apparent molecular mass of 35 to 40 kDa due to glycosylation. M-CSF is a potent hematopoietic factor produced by a variety of cells including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts, and osteoblasts. It is a key regulator of cellular proliferation, differentiation, and survivial of blood monocytes, tissue macrophages, and their progenitor cells. This cytokine is produced in a human cell expression system with serum-free, chemically defined media.

Technical Specifications:

• Species: Human
• Expression: HEK293 Cell Expressed
• Activity: Typically ≤ 4 ng/mL EC50
• Purity: >95%
• Endotoxin: <1 EU/µg
• Molecular Mass: 35 and 40 kDa, homodimer, glycosylated
• Formulation: 1x PBS

Purity Confirmation:

The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Activity Assay:

The activity was determined by the dose- dependent stimulation of the proliferation of murine M-NFS-60 cells (Mouse Myeloid Leukemia indicator cell line).

Reconstitution Buffer:

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Synonyms: Macrophage Colony Stimulating Factor, CSF-1, MGI-IM MCSF, M CSF, CSF1, CSF 1, MGIIM, MGI IM