Products : Recombinant Human IFN gamma

Products > Product Cytokines > IFN Gamma 

Recombinant Human IFN gamma

Product Description:

• Animal-derived product free
• High Activity
• Lyophilized and Carrier Free (CF)
• Glycosylated

Recombinant Human Interferon gamma (IFN γ), is a cytokine also known as type II interferon that provides immunity against bacterial, viral and protozoan infections. In its active form, IFN γ occurs as a glycosylated, non-covalently linked homodimer of 29-32 kDa subunits. It is produced by a number of immune cell types such as the natural killer cells, natural killer T cells, and effector lymphocyte T cells following antigenic and inflammatory triggers. Following secretion, the IFN γ dimer binds to its receptor which has two subunits: IFN-γR1 which is the ligand-binding chain (α chain) and IFN-γR2, the signal-transducing chain (β chain). The activation of the IFN γ receptor leads to activation of the JAK/STAT pathway which in turn activates IFN γ responsive genes. While IFN γ can inhibit viral replication, it also works as an immune-modulator and immune-stimulator by increasing surface expression of class I MHC proteins.

Technical Specifications:

• Species: Human
• Expression: HEK293 Cell Expressed
• Activity: Typically ≤ 0.05 ng/mL EC50
• Purity: >95%
• Endotoxin: <1 EU/μg
• Molecular Mass: 29-32 kDa
• Formulation: 1x PBS

Purity Confirmation:

The protein was resolved by SDS-polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Activity Assay:

The activity was determined by dose dependent inhibition of proliferation of HT-29 cells.

Reference:

1. Billiau A & Matthys P. (2009) Interferon-gamma: a historical perspective. Cytokine Growth Factor Rev 20(2): 97–113.
2. Schroder K et al. (2004) Interferon-gamma: an overview of signals, mechanisms and functions. J Leukoc Biol 75(2): 163–89

Reconstitution Buffer:

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1x PBS containing 0.1% endotoxin-free recombinant human albumin (HSA).

Synonyms: IFNgamma