Products : Recombinant Human FLT3 Ligand

Products > Product Cytokines > FLT3Ligand

Recombinant Human FLT3 Ligand

Product Description:

• Endotoxin-free
• Animal-derived product free
• Available in Bulk
• Lyophilized and Carrier Free (CF)
• Authentic Glycosylation

Xeno-free Recombinant Human FLT3 Ligand^HuXp is expressed from human 293 cells as a monomeric glycoprotein with an apparent molecular mass of 24 to 30 kDa. This cytokine is produced in a serum-free, chemically defined media. The molecular mass is a narrower range than reported when this cytokine is expressed in insect Sf21 cells (17 to 30 kDa), emphasizing the difference in glycosylation in different expression systems. FLT3 Ligand is a growth factor that regulates proliferation of early hematopoietic cells. FLT3 Ligand binds to cells expressing the tyrosine kinase receptor FLT3. FLT3 Ligand by itself does not stimulate proliferation of early hematopoietic cells, but synergizes with other CSFs and interleukins to induce growth and differentiation.

Technical Specifications:

• Species: Human
• Expression: HEK293 Cell Expressed
• Activity: Typically ≤0.8 ng/mL EC50
• Purity: >95%
• Endotoxin: <1 EU/µg
• Molecular Mass: 24 to 30 kDa, monomer, glycosylated
• Formulation: 1x PBS

Purity Confirmation:

The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Activity Assay:

The activity was determined by the dose- dependent stimulation of the proliferation of the human acute myeloid leukemia cell line OCI-AML5.

Reconstitution Buffer:

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile water containing 0.1% endotoxin-free recombinant human serum albumin (HSA)

Synonyms: Fms-related tyrosine kinase 3 ligand Fms-related tyrosine kinase3 ligand, Fms-related tyrosine kinase-3 ligand