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Recombinant Human FGF-Basic TS™ (Fibroblast Growth Factor, Thermostable)

Product Description:

• Endotoxin-free
• Animal-derived product free
• Available in Bulk
• Lyophilized and Carrier Free (CF)

Recombinant human FGFbasic-TS™ is expressed in human 293 cells. FGFbasic is a heparin-binding growth factor and critical for human embryonic stem cells to remain in an undifferentiated state during cell culture. FGFbasic-TS™ is a protein engineered FGFbasic to enchance its thermo- stability without modification of biological function. FGFbasic-TS™ consists of 154 amino acids as a 17 kDa, non-glycosylated monomer.

Technical Specifications:

• Species: Human
• Expression: HEK293 Cell Expressed
• Activity: Typically ≤0.5 ng/mL EC50
• Purity: >95%
• Endotoxin: <1 EU/µg
• Molecular Mass: 17 kDa, monomer, non-glycosylated
• Formulation: 1x PBS

Purity Confirmation:

The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Activity Assay:

The activity was determined by the dose- dependent stimulation of the proliferation of the Balb/c 3T3 cell line.

Reconstitution Buffer:

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Synonyms: Fibroblast Growth Factor-basic, FGF-2, HBGF-2, Prostatropin FGFbasicTS, FGFbasic TS, FGF2, FGF 2, HBGF2, HBGF, FGFbasic-TS