Products : Recombinant Human BMP-7

Products > Product Cytokines > BMP-7

Recombinant Human BMP-7

Product Description:

• Endotoxin-free
• Animal-derived product free
• Available in Bulk
• Lyophilized and Carrier Free (CF)
• Glycosylated disulfide–linked homodimer

Xeno-free Recombinant Human BMP-7^HuXp is expressed in human 293 cells as a disulfide linked homodimeric glycoprotein with an apparent molecular mass of 29 kDa. This cytokine is produced in a serum-free, chemically defined media. Recombinant Human BMP-7 is a homodimeric glycoprotein consisting of two 117 amino acid subunits, which correspond to amino acid residues 315 to 431 of the full-length BMP-7 precursor. The protein encoded by this gene is a member of the TGF-beta superfamily. Like other members of the Bone Morphogenetic Protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage.

Technical Specifications:

• Species: Human
• Expression: HEK293 Cell Expressed
• Activity: Typically ≤ 100 ng/mL EC50
• Purity: >95%
• Endotoxin: <1 EU/µg
• Molecular Mass: 29 kDa, homodimer, glycosylated
• Formulation: 2x PBS + 20% Ethanol

Purity Confirmation:

The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Activity Assay:

The activity was determined by the dose dependent induction of alkaline phosphatase production in the ATDC-5 cell line (Mouse chondrogenic cell line).

Reconstitution Buffer:

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile water containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Synonyms: OP-1  BMP 7, BMP7, OP 1, OP7