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IL-34 (Interleukin-34)

Product Description:

IL-34 is a homodimeric cytokine that is expressed in a range of tissues that include spleen, heart, brain, liver, kidney, lung, ovary, thymus, testis, small intestine, prostate and colon. IL-34 is a ligand for colony-stimulating factor-1 receptor (CSF1R), which also binds to CSF-1. It specifically binds CD14+ monocytes, promotes survival and proliferation of human peripheral blood monocytes, and stimulates macrophage colony formation by human bone marrow cells. IL-34, like human CSF-1, stimulates phosphorylation of MAPK1/ERK2 and MAPK3/ERK1. Recombinant Human IL-34 is a 52.5 kDa homodimeric glycoprotein consisting of 460 amino acid residues, including a C-terminal His-Tag.

Technical Specifications:

• Species: Human
• Expression: E. coli Cell Expressed
• Activity: Typically ≤0.5 ng/mL ED50
• Purity: >95%
• Endotoxin: <1.0 EU/μg
• Molecular Mass: 52.5 kDa, homodimeric, glycoprotein
• Formulation: 20 mM Sodium Citrate, pH 3.0 + 75mM NaCl
• Country of Origin: USA

Purity Confirmation:

This was determined by SDS-PAGE gel and HPLC analysis.

Activity Assay:

Measured by its ability to induce secretion of MCP-1 from PBMCs.

AA Sequence:

Reconstitution Buffer:

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex.

Storage:

This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% HSA) and store in working aliquots at -20°C to -80°C.