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DKK-1 (Dickkopf-related protein-1)

Product Description:

DKK-1 is a member of the DKK protein family which also includes DKK-2, DKK-3 and DKK-4. DKK-1 was originally identified as a Xenopus head-forming molecule that behaves as an antagonist for Wnt signaling. Subsequent studies have shown that DKK-1 and DKK-4 play an important regulatory role in the Wnt/β-catenin signaling pathway by forming inhibitory complexes with LDL receptor-related proteins 5 and 6 (LRP5 and LRP6), which are essential components of the Wnt/β-catenin signaling system. LPR5 and LPR6 are single-pass transmembrane proteins that appear to act as co-receptors for Wnt ligands involved in the Wnt/β-catenin signaling cascade. It has been suggested that by inhibiting Wnt/β-catenin signaling, which is essential for posterior patterning in vertebrates, DKK-1 permits anterior development. This notion is supported by the finding that mice deficient of DKK-1 expression lack head formation and die during embryogenesis. Mature human DKK-1 expressed in HEK293 cells is a 35-40 kDa glycoprotein containing 235 amino acid residues.

Technical Specifications:

  • Species: Human
  • Expression: HEK293 Cell Expressed
  • Purity: ≥97%
  • Endotoxin: <1.0 EU/μg
  • Molecular Mass: 25.8 kDa
  • Formulation: 1x PBS, pH 7.4
  • Country of Origin: USA

Purity Confirmation:

This was determined by SDS-PAGE gel and HPLC analysis.

Activity Assay:

Determined by its ability to inhibit the proliferation of HCT116 colorectal carcinoma cells. Approximately 40% growth inhibition was achieved at a DKK-1 concentration of 200ng/ml.

AA Sequence:

TLNSVLNSN IKNLPPPLGG AAGHPGSAVS
AAPGILYPGG NKYQTIDNYQ PYPCAEDEEC
GTDEYCASPT RGGDAGVQIC LACRKRRKRC
MRHAMCCPGN YCKNGICVSS DQNHFRGEIE
ETITESFGND HSTLDGYSRR TTLSSKMYHT
KGQEGSVCLR SSDCASGLCC ARHFWSKICK
PVLKEGQVCT KHRRKGSHGL EIFQRCYCGE
GLSCRIQKDH HQASNSSRLH TCQRH

Reconstitution Buffer:

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex.

Storage:

For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% HSA) and store in working aliquots at -20°C to -80°C.

Cat #: Size Price
HZ-7280 2 µg $82
HZ-7281 10 µg $201
HZ-7282 50 µg $1,071
HZ-7283 100 µg $3,214
HZ-7133 250 µg $5,356

XKine™ proteins are expressed in several expression systems

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