G4 Dendritic Cell Generation Xeno-Free HumanKine Cytokine Cocktail
- Produces high quality dendritic cells
- Yields are equal to or better than standard protocols
- Contains 1 vial of G4 DC HumanKine Cytokine Cocktail
- No cytokine aliquoting required
- Xeno and Animal-derived component free
Xeno free, animal free, recombinant human HumanKine GM-CSF from HEK293 cells
Xeno free, animal free, recombinant human HumanKine IL-4 from HEK293 cells
HumanKine GM-CSF Typical ED50 range = 0.4 to 2.0 ng/mL, determined by the dose dependent proliferation of TF-1 cells.
HumanKine IL-4 Typical ED50 range = 0.1 to 0.5 ng/mL, determined by the dose dependent proliferation of TF-1 cells.
Briefly centrifuge cytokine cocktail prior to opening to draw contents to the bottom of the vial. Reconstitute the cytokine cocktail in 100 (500) uL sterile 1x PBS. This stock solution can be added to 100 (500) mL of G4 DC basal medium. Complete medium (cytokine cocktail added to media) is stable at 4ºC for 30 days when protected from light. It is recommended to aliquot the complete medium into required working amounts to avoid exposing the complete medium to 37ºC multilple times.
Storage and Stability:
Lyophilized, Xeno-free, Animal free recombinant HumanKine HSA as a carrier.
Less than 1 EU/ug
A certificate of analysis is available on request.
The protein was resolved by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.