TNF beta (Tumor Necrosis Factor beta)
TNF-β is a potent mediator of inflammatory and immune responses. It belongs to the TNF family of ligands, and signals through TNFR1 and TNFR2. TNF-β is produced by activated T and B lymphocytes, and has similar activities to TNF-α. Like TNF-α, TNF-β is involved in the regulation of various biological processes, including cell proliferation, differentiation, apoptosis, lipid metabolism, coagulation, and neurotransmission. TNF-β is secreted as a soluble polypeptide, but can form heterotrimers with lymphotoxin-β, which effectively anchors the TNF-β to the cell surface. TNF-β is cytotoxic to a wide range of tumor cells. Recombinant Human TNF-β is a secreted 172 amino acid protein (18.6 kDa) which forms an active non-disulfide-linked homotrimer structure in solution.
- Species: Human
- Expression: E. coli Cell Expressed
- Activity: Typically ≤0.05 ng/ml ED50
- Purity: ≥98%
- Endotoxin: <1.0 EU/μg
- Molecular Mass: 18.6 kDa
- Country of Origin: USA
This was determined by SDS-PAGE gel and HPLC analysis.
The ED50 was determined by the cytolysis of murine L929 cells in the presence of Actinomyocin D.
Centrifuge the vial prior to opening.Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex.
This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% HSA) and store in working aliquots at -20°C to -80°C.