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TNF beta (Tumor Necrosis Factor beta)

 Product Description:

TNF-β is a potent mediator of inflammatory and immune responses. It belongs to the TNF family of ligands, and signals through TNFR1 and TNFR2. TNF-β is produced by activated T and B lymphocytes, and has similar activities to TNF-α. Like TNF-α, TNF-β is involved in the regulation of various biological processes, including cell proliferation, differentiation, apoptosis, lipid metabolism, coagulation, and neurotransmission. TNF-β is secreted as a soluble polypeptide, but can form heterotrimers with lymphotoxin-β, which effectively anchors the TNF-β to the cell surface. TNF-β is cytotoxic to a wide range of tumor cells. Recombinant Human TNF-β is a secreted 172 amino acid protein (18.6 kDa) which forms an active non-disulfide-linked homotrimer structure in solution.

Technical Specifications:

  • Species: Human
  • Expression: E. coli Cell Expressed
  • Activity: Typically ≤0.05 ng/ml ED50
  • Purity: ≥98%
  • Endotoxin: <1.0 EU/μg
  • Molecular Mass: 18.6 kDa
  • Country of Origin: USA

Purity Confirmation:

This was determined by SDS-PAGE gel and HPLC analysis.

Activity Assay:

The ED50 was determined by the cytolysis of murine L929 cells in the presence of Actinomyocin D.

AA Sequence:

MLPGVGLTPS AAQTARQHPK MHLAHSTLKP
AAHLIGDPSK QNSLLWRANT DRAFLQDGFS
LSNNSLLVPT SGIYFVYSQV MVYPGLQEPW
LHSMYHGAAF QLTQGDQLST HTDGIPHLVL
SPSTVFFGAF AL

Reconstitution Buffer:

Centrifuge the vial prior to opening.Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex.

Storage:

This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% HSA) and store in working aliquots at -20°C to -80°C.

Cat #: Size Price
HZ-7146 5 µg $82
HZ-7147 20 µg $201

XKine™ proteins are expressed in several expression systems

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