TGF beta 1 (Transforming Growth Factor beta 1)
The three mammalian isoforms of TGF beta, TGF beta 1, beta 2, and beta 3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility, as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes, including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes, which are stored at the cell surface and in the extracellular matrix. The release of the biologically active TGF beta isoform from a latent complex involves proteolytic processing of the complex and /or induction of conformational changes by proteins such as thrombospondin-1. TGF beta 1 is the most abundant isoform secreted by almost every cell type. It was originally identified for its ability to induce phenotypic transformation of fibroblasts, and recently it has been implicated in the formation of skin tumors.
- Species: Human
- Expression: CHO Cell Expressed
- Activity: Typically ≤0.05 ng/ml ED50
- Purity: ≥98%
- Endotoxin: <1.0 EU/μg
- Molecular Mass: 25.0 kDa
- Country of Origin: USA
This was determined by SDS-PAGE gel and HPLC analysis.
Determined by TGF-β1’s ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells.
Centrifuge the vial prior to opening. Reconstitute in 10mM Citric Acid, pH 3.0 to a concentration of 0.1-1.0 mg/ml. Do not vortex.
For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% HSA) and store in working aliquots at -20°C to -80°C.