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IL-35 (Interleukin-35)

Product Description:

Interleukin-35 is a glycosylated, disulfide-linked, heterodimeric protein consisting of the p35 subunit from IL-12 (IL-12α) and the β subunit from IL-27 (EBI3). IL-35 can be expressed by regulatory T-cells (Tregs), macrophages, and certain trophoblast and dendritic cells. It is induced in response to inflammation, and generally acts as an inflammation suppressor. IL-35 suppresses inflammation by exerting multiple activities, including the induction of regulatory T-cells and the suppression of Th17 cells. Recombinant Human IL-35 produced from HEK293 cells is a glycosylated, heterodimeric protein that migrates as a diffuse band centered at an apparent molecular weight of about 60 kDa by SDS-PAGE analysis under non-reducing conditions.

Technical Specifications:

  • Species: Human
  • Expression: HEK293 Cell Expressed
  • Activity: Typically ≤0.5 ng/mL ED50
  • Purity: ≥90%
  • Endotoxin: <1.0 EU/μg
  • Molecular Mass: 45.8 kDa
  • Formulation: 10 mM Sodium Phosphate, pH 7.0 + 0.1% CHAPS
  • Country of Origin: USA

Purity Confirmation:

This was determined by SDS-PAGE gel and HPLC analysis.

Activity Assay:

Measured by its ability to inhibit proliferative activity of PBMCs.

AA Sequence:

Alpha Subunit:

RNLPVATPDP GMFPCLHHSQ NLLRAVSNML
QKARQTLEFY PCTSEEIDHE DITKDKTSTV
EACLPLELTK NESCLNSRET SFITNGSCLA
SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN
AKLLMDPKRQ IFLDQNMLAV IDELMQALNF
NSETVPQKSS LEEPDFYKTK IKLCILLHAF
RIRAVTIDRV MSYLNAS

Beta Subunit:

RKGPPAALTL PRVQCRASRY PIAVDCSWTL
PPAPNSTSPV SFIATYRLGM AARGHSWPCL
QQTPTSTSCT ITDVQLFSMA PYVLNVTAVH
PWGSSSSFVP FITEHIIKPD PPEGVRLSPL
AERQLQVQWE PPGSWPFPEI FSLKYWIRYK
RQGAARFHRV GPIEATSFIL RAVRPRARYY
VQVAAQDLTD YGELSDWSLP ATATMSLGK

Reconstitution Buffer:

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex.

Storage:

For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Cat #: Size Price
HZ-7066 2 µg $82
HZ-7067 10 µg $201

XKine™ proteins are expressed in several expression systems

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