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IL-1 alpha (Interleukin-1 alpha)

Product Description:

IL-1 alpha possesses a wide spectrum of metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. It binds to the interleukin-1 receptor. IL-1 alpha is constitutively produced by epithelial cells. It is found in substantial amounts in normal human epidermis and is distributed in a 1:1 ratio between living epidermal cells and stratum corneum. The constitutive production of large amounts of IL-1 alpha precursor by healthy epidermal keratinocytes interfere with the important role of IL-1 alpha in immune responses, assuming skin as a barrier, which prevents the entry of pathogenic microorganisms into the body. In vitro, IL-1 alpha possesses biological effect on cells in the picomolar to femtomolar range. In vivo, shortly after an onset of an infection into organism, IL-1 alpha activates a set of immune system response processes.

Technical Specifications:

  • Species: Human
  • Expression: HEK293 Cell Expressed
    • Activity: Assay 1: Typically 0.5-5 μg/mL ED50
    • Assay 2: Typically <1 pg/mL ED50
  • Purity: >95%
  • Endotoxin: <1.0 EU/μg
  • Molecular Mass: 18 kDa
  • Formulation: PBS, pH 7.4
  • Country of Origin: USA

Purity Confirmation:

This was determined by SDS-PAGE.

Activity Assay:

Assay#1: Measured by its binding ability in a functional ELISA.

Assay#2: The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse D10S cells.

Reconstitution Buffer:

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage:

Lyophilized Protein should be stored at -20° or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20° or -70°. Avoid repeated freeze-thaw cycles.

Cat #: Size Price
HZ-7363 10 µg $226
HZ-7212 50 µg $576
HZ-7213 1000 µg $7,043

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