IL-1 alpha (Interleukin-1 alpha)
IL-1 alpha possesses a wide spectrum of metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. It binds to the interleukin-1 receptor. IL-1 alpha is constitutively produced by epithelial cells. It is found in substantial amounts in normal human epidermis and is distributed in a 1:1 ratio between living epidermal cells and stratum corneum. The constitutive production of large amounts of IL-1 alpha precursor by healthy epidermal keratinocytes interfere with the important role of IL-1 alpha in immune responses, assuming skin as a barrier, which prevents the entry of pathogenic microorganisms into the body. In vitro, IL-1 alpha possesses biological effect on cells in the picomolar to femtomolar range. In vivo, shortly after an onset of an infection into organism, IL-1 alpha activates a set of immune system response processes.
- Species: Human
- Expression: HEK293 Cell Expressed
- Activity: Assay 1: Typically 0.5-5 μg/mL ED50
- Assay 2: Typically <1 pg/mL ED50
- Purity: >95%
- Endotoxin: <1.0 EU/μg
- Molecular Mass: 18 kDa
- Formulation: PBS, pH 7.4
- Country of Origin: USA
This was determined by SDS-PAGE.
Assay#1: Measured by its binding ability in a functional ELISA.
Assay#2: The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse D10S cells.
See Certificate of Analysis for reconstitution instructions and specific concentrations.
Lyophilized Protein should be stored at -20° or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20° or -70°. Avoid repeated freeze-thaw cycles.