IFN beta (Immune Interferon beta)
Proteins of this family play an important role in inducing non-specific resistance against a broad range of viral infections. They also affect cell proliferation and modulate immune responses. Produced by peripheral blood leukocytes and lymphoblastoid cells, IFN alpha is an acid-stable molecule that signals through IFN-α/βR, which is also used by IFN beta. Both IFNs have similar anti-viral activity and regulate expression of MHC class I antigens. IFN alpha contains four highly conserved cysteine residues that form two disulfide bonds, one of which is necessary for biological activity.
- Species: Human
- Expression: CHO Cell Expressed
- Activity: Assay 1: Typically 20-30 ng/ml ED50
- Assay 2: Typically ≤ 0.1 ng/ml
- Purity: ≥95%
- Endotoxin: <1.0 EU/μg
- Molecular Mass: 22.3 kDa
- Formulation: 10 mM Sodium Citrate, 100 mM Sodium Chloride + 10 mM Sucrose
- Country of Origin: USA
This was determined by SDS-PAGE gel and HPLC analysis.
Assay#1: Determined by its ability to induce apoptosis in HeLa cells.
Assay#2: Determined by a cytotoxicity assay using human TF-1 cells.
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 1.0 mg/ml. Do not vortex.
This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% HSA) and store in working aliquots at -20°C to -80°C.