Products

Products > XKine Products > FGFbasic

FGFbasic (Fibroblast Growth Factor basic)

Product Description:

FGFbasic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGFbasic is a non-glycosylated, heparin-binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland, liver, monocytes, epithelial cells and endothelial cells. FGFbasic signals through FGFR 1b, 1c, 2c, 3c and 4. Recombinant Human FGFbasic is a 17.2 kDa protein consisting of 154 amino acid residues.

Technical Specifications:

  • Species: Human
  • Expression: E. coli Cell Expressed
  • Activity:
    • Assay 1: Typically ≤0.5 ng/mL ED50
    • Assay 2: Typically ≤0.1 ng/mL ED50
  • Purity: ≥95%
  • Endotoxin: <1.0 EU/μg
  • Molecular Mass: 17.2 kDa
  • Formulation: 5 mM Tris, pH 7.6 + 150 mM NaCl
  • Country of Origin: USA

Purity Confirmation:

This was determined by SDS-PAGE gel and HPLC analysis.

Activity Assay:

Assay#1: Determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors.

Assay#2: Determined by a cell proliferation assay using balb/c 3T3 cells.

AA Sequence:

AAGSITTLPA LPEDGGSGAF PPGHFKDPKR
LYCKNGGFFL RIHPDGRVDG VREKSDPHIK
LQLQAEERGV VSIKGVCANR YLAMKEDGRL
LASKCVTDEC FFFERLESNN YNTYRSRKYT
SWYVALKRTG QYKLGSKTGP GQKAILFLPM
SAKS

Reconstitution Buffer:

Centrifuge the vial prior to opening. Reconstitute in 5mM Tris, pH 7.6, to a concentration of 0.1-1.0 mg/ml. Do not vortex.

Storage:

For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. Avoid repeated freeze-thaw cycles.

Cat #: Size Price
HZ-7021 10 µg $82
HZ-7022 50 µg $201
HZ-7023 100 µg $221
HZ-7024 250 µg $288
HZ-7025 500 µg $494
HZ-7026 1000 µg $824

XKine™ proteins are expressed in several expression systems

If your protein is not listed please inquire. Good possibility we might have it.

Click to Inquire