FGFbasic (Fibroblast Growth Factor basic)
FGFbasic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGFbasic is a non-glycosylated, heparin-binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland, liver, monocytes, epithelial cells and endothelial cells. FGFbasic signals through FGFR 1b, 1c, 2c, 3c and 4. Recombinant Human FGFbasic is a 17.2 kDa protein consisting of 154 amino acid residues.
- Species: Human
- Expression: E. coli Cell Expressed
- Assay 1: Typically ≤0.5 ng/mL ED50
- Assay 2: Typically ≤0.1 ng/mL ED50
- Purity: ≥95%
- Endotoxin: <1.0 EU/μg
- Molecular Mass: 17.2 kDa
- Formulation: 5 mM Tris, pH 7.6 + 150 mM NaCl
- Country of Origin: USA
This was determined by SDS-PAGE gel and HPLC analysis.
Assay#1: Determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors.
Assay#2: Determined by a cell proliferation assay using balb/c 3T3 cells.
Centrifuge the vial prior to opening. Reconstitute in 5mM Tris, pH 7.6, to a concentration of 0.1-1.0 mg/ml. Do not vortex.
For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. Avoid repeated freeze-thaw cycles.