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FGFacidic (Fibroblast Growth Factor acidic)

Product Description:

FGFacidic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGFacidic is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. FGFacidic has the ability to signal through all the FGF receptors. Recombinant Human FGFacidic is a 16.8 kDa protein consisting of 141 amino acid residues.

Technical Specifications:

  • Species: Human
  • Expression: E. coli Cell Expressed
  • Activity:
    • Assay 1:Typically ≤10 ng/mL ED50
    • Assay 2: Typically ≤0.5 ng/mL ED50
  • Purity: ≥95%
  • Endotoxin: <1.0 EU/μg
  • Molecular Mass: 16.8 kDa
  • Formulation: 5 mM Sodium Phosphate, pH 8.0 + 100 mM NaCl
  • Country of Origin: USA

Purity Confirmation:

This was determined by SDS-PAGE gel and HPLC analysis.

Activity Assay:

Assay#1: Determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors.

Assay#2: Determined by a cell proliferation assay using balb/c 3T3 cells.

AA Sequence:

MFNLPPGNYK KPKLLYCSNG GHFLRILPDG
TVDGTRDRSD QHIQLQLSAE SVGEVYIKST
ETGQYLAMDT DGLLYGSQTP NEECLFLERL
EENHYNTYIS KKHAEKNWFV GLKKNGSCKR
GPRTHYGQKA ILFLPLPVSS D

Reconstitution Buffer:

Centrifuge the vial prior to opening. Reconstitute in 5mM Sodium Phosphate, pH 8.0 to a concentration of <0.5 mg/ml. Do not vortex.

Storage:

This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% HSA) and store in working aliquots at -20°C to -80°C.

Cat #: Size Price
HZ-7015 10 µg $82
HZ-7016 50 µg $201
HZ-7017 100 µg $309
HZ-7018 250 µg $541
HZ-7019 500 µg $927
HZ-7020 1000 µg $1,545

XKine™ proteins are expressed in several expression systems

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