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Wnt3A (Recombinant Human)

Product Description:

  • Animal-derived product free
  • High Activity
  • Lyophilized and Carrier Free (CF)

Wnt3AHuXp is a member of the Wnt gene family that consists of structurally linked genes that encode secreted signaling proteins. These proteins are associated with oncogenesis and in numerous developmental processes. These processes include regulation of cell fate and patterning during embryogenesis. Human Wnt3A is a protein which shows 84% identity relative to other human Wnt3 proteins. Wnt3A plays a key role in maintaining the integrity of embryonic and adult tissues. The post-translational glycosylation and acylation of Wnts are essential for their efficient secretion and biological activity.

Technical Specifications:

  • Species: Human
  • Expression: HEK293 Cell Expressed
  • Activity: Typically ≤ 20 ng/mL EC50
  • Purity: >90%
  • Endotoxin: <1 EU/μg
  • Molecular Mass: 38-42 kDa
  • Formulation: 1x PBS pH 7.4 + 500mM NaCl +1% CHAPS

Purity Confirmation:

The protein was resolved by SDS-polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Activity Assay:

The activity was determined by dose dependent Alkaline Phosphatase production in MC3T3 E1 mouse preosteoblast cells(1) and stabilization of beta catenin in mouse L929 cells(2).

Reconstitution Buffer:

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to ≤100μg/mL in sterile 1x PBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

References:

1) Walter, K., and Schutt, C., in Methods of Enzymatic Analysis, Bergmeyer, H.U. ed., 2nd ed., Volume II, Academic Press, Inc. (New York, NY:1974), 860- 864.
2) van Noort M, et al.,2002, J Biol Chem, 277(20), 17901-5.

(Complimentary HSA will be provided with shipment)

 

Synonyms: Wnt3A

Cat #: Size Price
HZ-1295  5 µg $278
HZ-1296 10 µg $399
HZ-1297 100 µg Inquire

Human Cell Expressed

Animal Component-Free, Xeno-Free Production