- Animal-derived product free
- Available in Bulk
- Lyophilized and Carrier Free (CF)
- Authentic Glycosylation
Xeno-free TPO is expressed from human 293 cells as a monomeric glycoprotein with an apparent molecular mass of 80 to 85 kDa. This cytokine is produced in a serum-free, chemically defined media. Production in human 293 cells offers authentic glycosylation, contributing to stability in cell growth media and other applications. C- terminal domain glycosylation is thought to be important for the secretion of TPO from cells and for survival of TPO in the circulation.
- Species: Human
- Expression: HEK293 Cell Expressed
- Activity: Typically ≤ 5 ng/mL EC50
- Purity: >95%
- Endotoxin: < 1 EU/µg
- Molecular Mass: 80 to 85 kDa, monomer, glycosylated.
- Formulation: 1x PBS
The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.
The activity was determined by the dose- dependent stimulation of the proliferation of MO7e cells.
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).
Synonyms: Thrombopoietin, Megakaryocyte colony-stimulating factor, c-MPL Ligand, MGDF cMPL Ligand, c MPL Ligand,