TGF beta 1 (Transforming Growth Factor Beta 1)
- Animal-derived product free
- Available in Bulk
- Lyophilized and Carrier Free (CF)
Xeno-free TGF beta 1 is expressed from human 293 cells as a mature disulfide-linked homodimer with an apparent molecular mass of 25 kDa. Tests indicate that TGF beta 1 is as efficient at inducing IL-17 expression in Th17 cells, as cytokine purified from human platelets. This product is produced in a human cell expression system with serum-free, chemically defined media.
- Species: Human
- Expression: HEK293 Cell Expressed
- Activity: Typically ≤0.5 ng/mL EC50
- Purity: >95%
- Endotoxin: <1 EU/µg
- Molecular Mass: 25 kDa, homodimer, nonglycosylated
- Formulation: 50 mM NaOAc pH 4.5
The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.
The activity was determined by the dose dependent inhibition of IL-4 induced proliferation of mouse HT-2 cells (BALB/c spleen activated by sheep erythrocytes in the presence of IL-2)..
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 4 mM HCl containing 0.1% endotoxin-free recombinant human serum albumin (HSA).
Synonyms: Transforming Growth Factor-β1, Differentiation inhibiting factor, Cartilage-inducing factor Transforming Growth Factorβ1, Transforming Growth Factor- beta;1