Noggin (Recombinant Human)
- Animal-derived product free
- Available in Bulk
- Lyophilized and Carrier Free (CF)
- Authentic Glycosylation
Xeno-free NogginHuXp is expressed in an engineered human 293 cell expression system with serum- free, chemically defined media. The protein is a highly stable, authentically glycosylated, disulfide linked dimer. Recombinant Human Noggin is a 46 kDa disulfide-linked homodimer (120-10C) consisting of two 206 amino acid polypeptide chains. Monomeric glycosylated Noggin migrates at an apparent molecular weight of approximately 28.0-33.0 kDa by SDS-PAGE analysis under reducing conditions.
- Species: Human
- Expression: HEK293 Cell Expressed
- Activity: Typically ≤ 25 ng/mL EC50
- Purity: >95%
- Endotoxin: <1 EU/μg
- Molecular Mass: 65 kDa, homodimer, glycosylated
- Formulation: 1x PBS
The protein was resolved by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.
The activity was determined by the dose dependent inhibition of rh-BMP-4 induced alkaline phosphate production by ATDC5 cells.
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).