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Noggin  (Recombinant Human)

Product Description:

  • Endotoxin-free
  • Animal-derived product free
  • Available in Bulk
  • Lyophilized and Carrier Free (CF)
  • Increased Stability, High Purity

Xeno-free Noggin is expressed in an engineered human 293 cell expression system with serumfree, chemically defined media. The protein is a highly stable, authentically glycosylated, disulfide linked dimer. Recombinant Human Noggin is a 46 kDa disulfide-linked homodimer (120-10C) consisting of two 206 amino acid polypeptide chains. Monomeric glycosylated Noggin migrates at an apparent molecular weight of approximately 28.0-33.0 kDa by SDS-PAGE analysis under reducing conditions.

Technical Specifications:

  • Species: Human
  • Expression: HEK293 Cell Expressed
  • Activity: Typically ≤ 25 ng/mL EC50
  • Purity: >95%
  • Endotoxin: <1 EU/μg
  • Molecular Mass: 65 kDa, homodimer, glycosylated
  • Formulation: 1x PBS

Purity Confirmation:

The protein was resolved by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Activity Assay:

The activity was determined by the dose dependent inhibition of rh-BMP-4 induced alkaline phosphate production by ATDC5 cells.

Reconstitution Buffer:

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Noggin

Cat #: Size Price
HZ-1118 10 µg $191
HZ-1120 100 µg $955
HZ-1085 1000 µg $3,469

Human Cell Expressed

Animal Component-Free, Xeno-Free Production