IL-7 (Interleukin 7)
- Animal-derived product free
- Lyophilized and Carrier Free (CF)
- Higher Activity
Xeno-free IL-7HuXp is expressed in Human 293 cells as a highly glycosylated protein. Under reducing conditions, IL-7 migrates as three separate bands as 19 kDa, 25 kDa and 30 kDa. In comparison IL-7 produced in an E. coli expression system have glycosylation and migrate as a single band at 17 kDa. IL-7 is a hematopoietic cytokine that is important for B and T cell development. When combined with other factors, IL-7 can also co-stimulate the proliferation of mature T cells. Production in human 293 cells uses a serum-free, chemically defined media and offers authentic glycosylation.
- Species: Human
- Expression: HEK293 Cell Expressed
- Activity: Typically ≤ 1 ng/mL EC50
- Purity: >95%
- Endotoxin: <1 EU/μg
- Molecular Mass: 19, 25, and 30 kDa, monomer, glycosylated
- Formulation: 1x PBS
The protein was resolved by SDS-polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.
The specific activity was determined by the dose- dependent stimulation of the proliferation of murine 2E8 cells.
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).