IL-27 (Recombinant Human)
- Animal-derived product free
- Available in Bulk
- High Activity
Xeno-free HumanKine® IL-27 is expressed in human 293 cells as a non-covalently bonded heterodimer cytokine composed of IL27p28 (25 kDa) and glycosylated EB13 (30 kDa). Production in human 293 cells uses a serum-free, chemically defined media and offers authentic glycosylation. Cytokines produced in non-human expression systems may have an artificial linker, a tag, and/or not human-like posttransational modifications.
- Species: Human
- Expression: HEK293 Cell Expressed
- Activity: Typically ≤12 ng/mL EC50
- Purity: >95%
- Endotoxin: <1 EU/μg
- Molecular Mass: 25 and 30 kDa heterodimer, glycosylated
- Formulation: 1x PBS
The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.
The specific activity was determined by the dose-dependent inhibition of HIV replication in human CD4+ T cells and in human monocyte-derived macrophages.Fakruddin, J.M., et al. Blood 109:1841.
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).
Synonyms: IL27, IL 27