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Products > Product Cytokines > IL-12

IL-12  (Interleukin 12)

Product Description:

  • Endotoxin-free
  • Animal-derived product free
  • Available in Bulk
  • Lyophilized and Carrier Free (CF)
  • Expressed as Disulfide-linked Heterodimer
  • Authentic Glycosylation

Xeno-free IL-12 is expressed in human 293 cells as a heterodimeric cytokine composed of two glycosylated and disulfide-linked subunits (p40 cysteine-linked to p35). Production in human 293 cells uses a serum-free, chemically defined media and offers authentic glycosylation. IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes, and connective tissue type mast cells.

Technical Specifications:

  • Species: Human
  • Expression: HEK293 Cell Expressed
  • Activity: Typically ≤2 ng/mL EC50
  • Purity: >95%
  • Endotoxin: <1 EU/μg
  • Molecular Mass: 57 kDa, heterodimer, glycosylated
  • Formulation: 1x PBS

Purity Confirmation:

The protein was resolved by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Activity Assay:

The activity was determined by the dose-dependent release of IFN-gamma from the human NK92 cell line in presence of 20 ng/mL rIL-2.

Reconstitution Buffer:

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

IL-12

Synonyms: NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF IL 12, IL12

Cat #: Size Price
HZ-1256 10 µg $226
HZ-1257 100 µg $1,910
HZ-1258 1000 µg $6,843

Human Cell Expressed

Animal Component-Free, Xeno-Free Production