IL-1 beta (Interleukin 1 beta)
- Animal-derived product free
- Available in Bulk
- Authentic Glycosylation
- Lyophilized and Carrier Free (CF)
Xeno-free IL-1 beta expressed in human 293 cells has a very high activity with a typical EC50 of ≤0.1 ng/mL using a bioassay to measure stimulation of the proliferation of mouse D10S cells. In comparison with the E. coli expressed protein, IL-1 beta is 50% more potent promoting human CD4+ T cell differentiation into Th17 cells. This cytokine is produced in a serum-free, chemically defined media.
- Species: Human
- Expression: HEK293 Cell Expressed
- Activity: Typically ≤0.1 ng/mL EC50
- Purity: >95%
- Endotoxin: <1 EU/µg
- Molecular Mass: 18 and 25 kDa, monomer, glycosylated
- Formulation: 1x PBS
The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.
The activity was determined by the dose- dependent stimulation of the proliferation of mouse D10S cells.
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).
Synonyms: Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF) Lymphocyte activating factor (LAF)