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GM-CSF (Granulocyte-macrophage colony-stimulating factor)

Product Description:

  • Endotoxin-free
  • Animal-derived product free
  • Available in Bulk
  • Higher Stability in Media
  • 10-Fold Higher Potency
  • Lyophilized and Carrier Free (CF

Xeno-free GM-CSF is produced in a human cell expression system in serum-free, chemically defined media. It has a demonstrated greater stability under cell culture conditions making it ideal for efficient generation of Human Dendritic Cells. (See the preferred, time saving G4 protocol available at www.HumanZyme.com). GM-CSF is a hematopoietic growth factor that stimulates the development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in endothelial cells, monocytes, fibroblasts, and T lymphocytes. Recombinant Human GM-CSF is a 15 to 36 kDa golbular protein consisting of 128 amino acids, containing two intramolecular disulfide bonds and two potential N-linked glycosylation sites.

Technical Specifications:

  • Species: Human
  • Expression: HEK293 Cell Expressed
  • Activity: Typically ≤2 ng/mL EC50
  • Purity: >95%
  • Endotoxin: <1 EU/µg
  • Molecular Mass: 15 to 36 kDa, monomer, glycosylated
  • Formulation: 1x PBS

Purity Confirmation:

The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Activity Assay:

The activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).

Reconstitution Buffer:

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

GM-CSF

Synonyms: Granulocyte/Macrophage Colony-Stimulating Factor, CSF-2, MGI-1GM, Pluripoietin-&alpha CSF2, CSF 2, MGI1GM, MGI 1GM

Cat #: Size Price
HZ-1002 10 µg $226
HZ-1173 100 µg $1,591
HZ-1082 1000 µg $3,469

Human Cell Expressed

Animal Component-Free, Xeno-Free Production