GM-CSF (Granulocyte-macrophage colony-stimulating factor)
- Animal-derived product free
- Available in Bulk
- Higher Stability in Media
- 10-Fold Higher Potency
- Lyophilized and Carrier Free (CF
Xeno-free GM-CSF is produced in a human cell expression system in serum-free, chemically defined media. It has a demonstrated greater stability under cell culture conditions making it ideal for efficient generation of Human Dendritic Cells. (See the preferred, time saving G4 protocol available at www.HumanZyme.com). GM-CSF is a hematopoietic growth factor that stimulates the development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in endothelial cells, monocytes, fibroblasts, and T lymphocytes. Recombinant Human GM-CSF is a 15 to 36 kDa golbular protein consisting of 128 amino acids, containing two intramolecular disulfide bonds and two potential N-linked glycosylation sites.
- Species: Human
- Expression: HEK293 Cell Expressed
- Activity: Typically ≤2 ng/mL EC50
- Purity: >95%
- Endotoxin: <1 EU/µg
- Molecular Mass: 15 to 36 kDa, monomer, glycosylated
- Formulation: 1x PBS
The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.
The activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).
Synonyms: Granulocyte/Macrophage Colony-Stimulating Factor, CSF-2, MGI-1GM, Pluripoietin-&alpha CSF2, CSF 2, MGI1GM, MGI 1GM