G4 Dendritic Cell Generation Kit 500 mL (Recombinant Human)
- Produces high quality dendritic cells
- Yields are equal to or better than standard protocols
- No medium change or addition required
- No cytokine aliquoting required
- Xeno and Animal-derived component free
- Human cell expressed growth factors included
HumanKine® G4 Dendritic Cell (DC) Generation Kit has been developed for the growth and generation of human dendritic cells (DCs). Using HumanKine G4 DC medium, human dendritic cells can be produced from peripheral blood mononuclear cells WITHOUT MEDIUM CHANGE and used either as immature DCs or further cultivated into mature DCs.
Protocol in Brief:
- Reconstitute Cytokine Cocktail
- Add Cocktail to Basal Medium
- Suspend monocytes in complete medium
- Incubate 7 days
- DO NOT CHANGE MEDIUM
- DO NOT ADD MEDIUM.
G4 DC Basal Medium
Growth Factor Cocktail
Kit includes all necessary medium and reagents (except beta-mercaptoethanol and Fetal Calf Serum)
100 mL HZ-9101
500 mL HZ-9501
NOTE: A 10 mL culture requires only 10 mL medium.(plating 3 to 5 x105 cells/mL) No medium addition or change is required.
Storage and Stability:
G4 DC Basal Medium: Store at 4ºC, protect from light. Medium is stable for six months from date of production.
G4 DC Cocktail (Lyophilized): Store at -80ºC. Cocktail is stable for twelve months from date of receipt.
Complete medium (G4 DC Cocktail added to G4 DC Basal Medium): Can be stored at 4ºC for up to 30 days in the dark.
A certificate of analysis is available on request.
Adding the Growth Factor Cocktail to the G4 DC Basal Medium produces G4 Dendritic Cell Generation Medium requiring no medium addition or change during the incubation period.
The resulting cells are monocyte-derived immature DCs that are highly phagocytic with the immature DC surface expression phenotype (CD14neg, CD83neg, CD80llow, CD86low, MHC Iint, and MHCint) and can migrate in response to CCR5 agonist (RANTES), but not to CCR7 agonists (ELC/SLC).
The protein was resolved by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.