G-CSF (Recombinant Human)
- Animal-derived product free
- Available in Bulk
- High Potency for Hematopoietic Cell Growth
- Authentic Glycosylation
Xeno-free G-CSFHuXp is expressed in human 293 cells as a monomeric glycoprotein with an apparent molecular mass of 21 to 25 kDa. This molecular mass is due to glycosylation, which is absent when this cytokine is expressed in E. coli. Glycosylation contributes to stability in cell growth media and other applications. This cytokine is produced in a serum-free, chemically defined media. The purity is greater than 95%.
- Species: Human
- Expression: HEK293 Cell Expressed
- Activity: Typically ≤0.1 ng/mL EC50
- Purity: >95%
- Endotoxin: <1 EU/μg
- Molecular Mass: 21 to 25 kDa, monomer, glycosylated
- Formulation: 1x PBS
The protein was resolved by SDS-polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.
The activity was determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells (Mouse Myeloid Leukemia indicator cell line).
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).
Synonyms: Granulocyte Colony-Stimulating Factor, CSF-3, MGI-1G, GM-CSF beta, Pluripoietin CSF3, CSF 3, MGI1G, MGI 1G, GMCSF beta, GM CSF beta