Cystatin C (Recombinant Human)
- Animal-derived product free
- Available in Bulk
- High Activity
Xeno-free Cystatin CHuXp (Cys-C) is expressed in human 293 cells as a monomer with an apparent molecular mass of 12 to 13 kDa. Native Cys-C in human urine is found in two different forms: one with pI 9.2 and the other with pI 7.8 by elimination of small basic peptides or amino acids from the N-terminal end of protein. Cystatin C has been studied for its role in predicting newonset or deteriorating cardiovascular disease. This cytokine is produced in a serum-free, chemically defined media.
- Species: Human
- Expression: HEK293 Cell Expressed
- Activity: ≤ 5 μM IC50
- Purity: >95%
- Endotoxin: <1 EU/μg
- Molecular Mass: 12 to 13 kDa, monomer, nonglycosylated
- Formulation: 1x PBS
The protein was resolved by SDS-polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.
The inhibitory function of Cystatin C on papin’s protease activity was measured by a colorimetric assay using L-BAPA as substrate. IC50 value was measured at 5 to 20 μg/mL (0.3 to 1.5 μM) with a range of 1.56μg/mL to 50μg/mL Cystatin C in presence of 0.55μM papain and 0.44μM L-BAPA.
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1x PBS.
Synonyms: Cystatin C Cystatin-C, CystatinC